Technical Specialist
Seetharamaiah (Ram) Attili, Ph.D., leverages his technical background in computer sciences, physics and bio physics, nanomaterials, and nanotechnology to assist with complex intellectual property matters.
Ram joined Finnegan with an extensive scientific research career in developing biophysical tools to understand the impact of force on molecular mechanisms of muscle growth. He established an instrumental setup to stimulate the muscle of Zebra fish larvae at the early stages of development. In an additional study, Ram evaluated the impact of drugs on length-dependant activation of human cardiac muscle. In his capacity as a research fellow, his primary tasks were to understand the molecular mechanism of phagocytosis using a custom build micropipette aspiration setup. Throughout his graduate studies, Ram has presented posters at various scientific conferences and meetings.
While obtaining his doctorate from Max-Planck Institute and University of the Basque Country, Ram's research focused on establishing a measurement method to investigate the mechanical properties of surface-confined Hyaluronan (HA)-rich films. To this end, he developed a research instrument combining two modern methods: colloidal probe atomic force microscopy (AFM) and reflection interference contrast microscopy (RICM), a technique applied to study the mechanical properties of films of end-grafted HA on a supported lipid bilayer. Ram also evaluated compression forces and elasticity of HA brushes and delved into the study of quartz crystal microbalance with dissipation monitoring (QCM-D) and Spectroscopic Ellipsometry as well as other time resolved surface sensitive techniques used to quantify film formation, their interaction with other HA binding proteins (aggrecan) and mechanics of the HA brush system. Apart from investigating mechanical properties of HA films, Ram applied existing polymer/polyelectrolyte theories to quantify and bolster his experimental findings and theoretical predictions.
As a masters student at Swiss Federal Institute of Technology, Ram developed protocols to fabricate porous waveguides for biosensing with the aid of clean room techniques.
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